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    赵亮, 崔玉山, 曾强, 王凌之, 张斌, 侯常春, 刘洪亮. 碘化物过量对人神经细胞的损伤作用及机制研究[J]. 环境卫生学杂志, 2016, 6(1): 16-19. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.004
    引用本文: 赵亮, 崔玉山, 曾强, 王凌之, 张斌, 侯常春, 刘洪亮. 碘化物过量对人神经细胞的损伤作用及机制研究[J]. 环境卫生学杂志, 2016, 6(1): 16-19. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.004
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    ZHAO Liang, CUI Yushan, ZENG Qiang, WANG Lingzhi, ZHANG Bin, HOU Changchun, LIU Hongliang. Excess Iodide Effects on Autophagy of Human Nerve Cells (SH-SY5Y)[J]. Journal of Environmental Hygiene, 2016, 6(1): 16-19. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.004
    Citation: ZHAO Liang, CUI Yushan, ZENG Qiang, WANG Lingzhi, ZHANG Bin, HOU Changchun, LIU Hongliang. Excess Iodide Effects on Autophagy of Human Nerve Cells (SH-SY5Y)[J]. Journal of Environmental Hygiene, 2016, 6(1): 16-19. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.004
    shu

    碘化物过量对人神经细胞的损伤作用及机制研究

    Excess Iodide Effects on Autophagy of Human Nerve Cells (SH-SY5Y)

      摘要
    • 摘要: 目的 探讨碘化物过量对人神经母细胞瘤细胞(SH-SY5Y)的损伤作用及其机制。方法 将体外培养的SH-SY5Y细胞用0(对照)、10、20、30 mmol/L碘化钾(KI)分别染毒处理24 h后,采用CCK-8法、单丹黄酰尸胺(MDC)染色法及Wsetern blot实验检测细胞存活率、自噬囊泡生成以及自噬相关蛋白表达情况。结果 与对照组相比较,30 mmol/L碘化物染毒组细胞存活率明显下降(P<0.05),自噬囊泡生成明显增多(P<0.05),自噬相关蛋白Beclin1表达明显升高(P<0.05)。结论 一定剂量的碘可降低神经细胞的存活率,诱导细胞自噬,碘化物过量对神经细胞的损伤作用可能与其诱导活化细胞自噬有关。

       

      Abstract: Objectives To investigate the damage of excess iodide on human neuroblastoma cells (SH-SY5Y)and its related mechanism.Methods SH-SY5Y cells were treated with potassium iodide (KI)at the concentrations of 0, 10, 20 and 30 mmol/L in the medium for 24 hours.The viability of cells, the generation of autophagic vesicles and the expression of Beclin1 were tested by CCK-8 assay, monodansylcadaverine (MDC)-staining assay and western blot assay.Results Compared with the control group, the viability of cells in the 30 mmol/L KI group was decreased obviously (P<0.05), the generation of autophagic vesicles was increased (P<0.05)and the expression of Beclin1 protein was higher significantly (P<0.05).Conclusions Excess iodide can notablely decrease the viability of SH-SY5Y cells, increase the generation of autophagic vesicles and increase the expression of Beclin1 protein in SH-SY5Y cells. These results suggested that the damage of nerve cell induced by excess iodide might be related to autophagy.

       

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