Determination of the 15+1 European Priority Polycyclic Aromatic Hydrocarbons in Vegetable and Animal Oil by Using Molecularly Imprinted Solid-phase Extraction Followed by Gas Chromatography-mass Spectrometry

Objectives A method based on molecularly imprinted solid-phase extraction and gas chromatography-mass spectrometry (MISPE-GC/MS) for the determination of 15+1 European priority polycyclic aromatic hydrocarbons in vegetable and animal oil has been developed.

Methods The oil samples were diluted with cyclohexane and cleansed up by molecularly imprinted solid-phase extraction catridge (MISPE). The fraction was concentrated to 200 μL for GC/MS analysis. The PAHs were separated on an Agilent J&W DB-EUPAH column(20 m×0.18 mm×0.14 μm), selected ion monitoring (SIM) mode was used and CHRD12, BaPD12 and DiPD14 were used as internal standards for quantification.

Results The detection limits of 16 PAHs were 0.25~0.86 μg/kg, The linear relation for all target compounds were in the range of 2~200 μg/L with a correlation coefficient over 0.99. The Intra-day precision is less than 15%. The recoveries of spiked samples at the level of 2, 10 and 20 μg/kg were in the rang of 37.90%~105.24%, the relative standard deviations were all less than 15%.

Conclusion The method was sensitive and selective.