Determination of Residual Content of Herbicides by the Method of Ultra-high Performance Liquid Chromatography-mass Spectrometry

Objectives To establish a method for detecting the residual content of several kinds of herbicides in water using ultra-high performance liquid chromatography-mass spectrometry.

Methods Water sample was purified and concentrated by Oasis HLB column and then eluted by acetonitrile. The determination by chromatography-mass spectrometry was performed on 1.7 μm C18 column, using acetonitrile, formic acid(0.1%) and water as mobile phase in linear gradient elution.

Results The method can detect 30 kinds of herbicides. The spiked recovery rate varied in the range from 76.2% to 101.3%, with relative standard deviation in the range of 3.2% to 9.3% (n=6). The limit of operation was 0.1~1.0 μg/L with the linear range varying in the range of 0.0 to 200 μg/L.

Conclusions The method is sensitive, specific and simple in operation. It can detect the residual content of 30 kinds of herbicides at the same time.