Objectives To develop a quantitative detection method for the isolation and culture of legionella in water and soil.
Methods Using a recovery of standard addition test to explore a detected conditions for quantitative isolation and culture, recovery rate, limit of detection and calculation mode.
Results The limits of detection for water and soil samples were 1 cfu/mL and 102 cfu/g, the recoveries were in the range of 81%-95% and 30%-90%, respectively.
Conclusions A quantitative detection method applied to isolate and culture legionella in water and soil was proposed in this study.
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