Objectives To observe the effect of 8 solubilizers/solvents on the detection of mutagenicity in Ames Test to provide reference for reasonable selection of solubilizers/solvents.
Methods Reverse mutation of Salmonella typhimurium test was used for testing the mutagenic effect of 8 solubilizers/solvents, including 95% ethanol, methanol, acetone, tween-20, tween-60, tween-80, DMSO and ethyl acetate on the results implemented according to the Ames Tests method in the Hygienic Standard for Cosmetics (2007).
Results Under the concentrations of not more than 5.0 mg/petri dish for tween-20, and the maximum dose of 100.0 mg/petri dish for other 7 solubilizers/solvents, the comparative judgement of revertant colonies of TA97, TA98, TA100 and TA102 strains were negative.
Conclusion As the maximum dose, 100.0 mg/petri dish for 95% ethanol, methanol, acetone, tween-60, tween-80, DMSO and ethyl acetate, and not more than 5.0 mg/petri dish for tween-20 could be used in Ames Test.
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