Objectives To determine the pollution of avian influenza virus in the environment of live poultry markets and poultry farms in Jinan in the spring of 2014, and to provide scientific strategies for the prevention and control of avian influenza in human.
Methods Samples were collected from the environment in live poultry markets and poultry farms, and the samples were tested by real-time fluorescence quantitative PCR.
Results No positive sample for H5, H9 and H7 was detected in poultry farms. The positive rate for H5N1 in poultry markets was 25.32%, and the positive rate for H5H1 in the excrement of poultry was 24.41%, in sewages was 25.00%, and on the surface of cages for keeping poultry was 26.92%.
Conclusions The pollution of H5N1 virus was various in different live poultry markets and poultry farms.