Expression of AC-cAMP-PKA Signal Transduction Pathway in Bone Tissue of Rats with Chronic Fluorosis

Objectives To observe the expression of AC, cAMP and PKA in bone tissue of rats with chronic fluorosis.

Methods Forty-eight Wistar rats at 2 weeks after weaning were randomly divided into 4 groups according to body weight:a control group (treated with tap water) and 3 NaF exposure groups (treated with NaF in drinking water at 50 mg/L, 150 mg/L and 250 mg/L).There were 6 female rats and 6 male rats in each group.The rats were executed after 6 months of treatment.The concentration of fluoride in bone tissue and urine, the incidence rate of dental fluorosis, and the levels of AC, cAMP and PKA in bone tissue were assayed.

Results The concentrations of fluoride in bone tissue, urine and tooth for each fluoride exposure group were significantly higher than the control group (P < 0.05).Compared with the control group, the incidence of dental fluorosis in all exposure groups was obviously higher in a dose-dependent and exposure time-dependent manner.There was no significant difference on AC, cAMP and PKA in the bone tissue of rats in each group (P > 0.05).

Conclusions There was no change on the levels of AC, cAMP in the bone tissue of rats caused by sodium fluoride in the present study.