Objectives To provide reference for developing and improving diagnostic kits by comparison and analysis of the detection effects of four kinds of diagnostic kits in practice.
Methods Real-time RT-PCR was employed to detect Influenza A H1N1 virus and SPASS 13.0 was applied to analyze data.
Results The positive rates of different diagnostic kits were different. Among them, the positive rates of kit A, kit C, and kit B were respectively 100%, 100% and 95%, while the kit D was merely 80%. Furthermore, tested by analysis of variance and SNK Multi-Analysis with singular index, the results showed that there were significant differences among all of the four kinds of diagnostic kits for each indicator and the amplification efficiency of which was obviously different.
Conclusions Kit A, B and C can be used for detection of Influenza A H1N1 virus. However, kit C was slightly higher sensitive and more suitable for preliminary screening. Furthermore, kit D needs further improving in technic.
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