Determination of trans, trans-muconic acid in urine by UPLC-MS/MS

Objective  To establish a method for determination of trans, trans-muconic acid in urine by UPLC-MS/MS.

Methods  Trans, trans-muconic acid which was labeled by the isotope was added to the urine samples. Then the samples were purified by solid phase extraction column and separated by Shield RP18 chromatographic column, and detected by the triple quadrupole mass spectrometry. The contents of trans, trans-muconic acid were quantified by the internal standard method.

Results  The linear range of trans, trans-muconic acid was 20.0-1 000.0 μg/L, with a correlation coefficient of 0.9997. The detection limit and the quantification limit were 1.10 μg/L and 3.30 μg/L, respectively. The spiked recovery rate of this method was 98.3%-101% at the spiked concentrations of 50.0, 200.0, and 800.0 μg/L.The within-run and between-run relative standard deviation(RSD) were 0.88%-2.79% and 3.38%-5.63%, respectively.

Conclusion  This method is suitable for the determination of trans, trans-muconic acid in urine.