Expression and Significance of LncRNA CTBP1-AS1 in Glycidyl Methacrylate-Induced Malignantly Transformed 16HBE Cells

Objective To investigate the expression and significance of long non-coding RNA (LncRNA) CTBP1-AS1 (CTBP1 antisense RNA 1) in glycidyl methacrylate (GMA)-induced malignantly transformed 16HBE cells.

Methods The 30th generation of malignantly transformed 16HBE cells (induced by 8 μg/mL GMA) and the same generation of control cells (treated with DMSO) were harvested. High-throughput LncRNA microarray analysis was used to measure the difference in the expression profile of LncRNA between the two groups. Strategies such as fold change analysis and neighboring gene analysis were used to preliminarily identify LncRNA CTBP1-AS1 and the most likely protein-coding gene CTBP1 in the malignantly transformed 16HBE cells. Whole genome expression profile microarray and quantitative real-time PCR (qPCR) were used to verify the relative expression of LncRNA CTBP1-AS1 and CTBP1 mRNA.

Results The result of LncRNA microarray analysis showed that the CTBP1-AS1 and CTBP1 mRNA in the GMA group were upregulated 2.20-fold and 1.26-fold, respectively, compared with those in the DMSO group; the result of qPCR showed that the expression of LncRNA CTBP1-AS1 in the GMA group was significantly higher than that in the DMSO group(91.70±0.70)×10^-5 vs (26.74±0.23)×10^-5, P < 0.05, which was consistent with the result of LncRNA microarray analysis.

Conclusion GMA can induce the upregulation of LncRNA CTBP1-AS1 expression in 16HBE cells, and LncRNA CTBP1-AS1 can be considered as a specific molecular marker in GMA-induced malignantly transformed 16HBE cells.