Study on the Level of Maternal Urinary 1-Hydroxypyrene and the Polymorphism of Maternal and Neonatal hOGG1 and XRCC1 Genes

Objectives To investigate the relationship between maternal polycyclic aromatic hydrocarbon(PAHs) exposure in Urumqi and the polymorphisms of maternal and neonatal hOGG1 and XRCC1 genes.

Methods A total of 115 maternal urine, venous blood and neonatal cord blood were collected from a hospital in Urumqi, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for gene locus polymorphism of maternal and neonatal hOGG1 and XRCC1. The use of enzymatic hydrolysis-liquid chromatography-mass spectrometry to detect internal exposure marker of PAHs, 1-OH-P, in the maternal urine.

Results The median level of 1-OH-Pconcentration in maternal urine was 0.340 (0.208~0.555) μg/mmol Cr. The maternal hOGG1326Ser/Cys genotype was different, and the concentration of 1-OH-Pin the body was also different (P < 0.05). There was a statistically significant difference in the concentrations of 1-OH-P in the mothers with neonates carrying different XRCC1399Arg/Gln genotypes(P < 0.05). There was no significant difference in the concentration of 1-OH-P between the parturient carrying different XRCC1399Arg/Gln genotypes (P>0.05). There was no significant difference in the concentration of 1-OH-Pin the mothers with neotates carrying different hOGG1326Ser/Cys genotypes (P>0.05).

Conclusions Compared with domestic related research, PAHs in parturient in Urumqi were at a high the exposure levels. Maternal bodies carrying the hOGG1326Ser/Cys gene are more susceptible to DNA damage in the high exposure environment of PAHs.