Objectives To establish a method for simultaneous determination of 60 kinds of antibiotics in water samples by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Methods After the pH value of water sample was adjusted to 2.0, the water sample was then concentrated and purified by an oasis HLB solid phase extraction column, analyzed by UPLC-MS/MS analysis and quantified by internal standard method.
Results A good linearity for the majority of antibiotics in water samples was in the range of (0.2~40) ng/L, with a correlation coefficient (R2) of ≥ 0.99, the limit of determination (LOD) was (0.001~0.6) ng/L and the limit of quantification (LOQ) was (0.004~1.9) ng/L.The spiked recovery of samples were in the range of 58.3%~127.7% at 3 spike levels (1, 4 and 20 ng/L).The relative standard deviations (RSDs) were in the range of 1.6%~13.9%.
Conclusions The established method was simple, rapid and accurate, which could be applied to the determination and quantification of the residue of antibiotics in water samples.
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