Objectives To develope a qualitative and quantitative method for the determination of 1-bromopropane metabolites (bromoacetone, 1-bromo-2-propanol) in urine.
Methods The exposure of mice to 1-bromine propane through respiratory tract was used to set up an animal model. Urine samples collected in headspace vials were processed with dynamic headspace method, which were determined qualitatively and quantitatively by GC-MS, and quantified by external standard method.
Results These chemical compounds in urine were concentrated and enriched by dynamic headspace method, and the target peaks were separated by a DB-5MS (30 m×0.25 mm×0.25 μm) capillary column. Electron Ionization (EI) was selected as an ion source, single ion monitoring (SIM) was used for the separation of bromoacetone and 1-bromo-2-propanol in mice urine, which were determined qualitatively and quantitatively by mass spectrometric method. The reproducibilities within-run were 2.8%~4.9% and the recoveries were 91.8%~109.6%. The determination rates of bromoacetone and 1-bromo-2-propanol in urine collected from exposed animals were 100%.
Conclusions The dynamic headspace-gas chromatography-tandem mass spectrometry could be used to qualitatively, quantitatively and accurately determine the level of 1-bromopropane metabolite (bromoacetone, 1-bromo-2-propanol) in urine. Bromoacetone and 1-bromo-2-propanol could be used as biomarkers for 1-bromopropane exposure.
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