Determination of Antibiotic Residues in Soil by UPLC-MS/MS

Objective  To develop a fast ultra performance liquid chromatography-tandem mass spectrometric method (UPLC-MS/MS) to determine Quinolones, Macrolides and Lincomycins residues in soil.

Methods  Samples were extracted ultrasonically by V (1:1 aeetonitrile and 0.1 mol/L EDTA) and purified by SAX and HLB tandem solid phase extraction column, separated by ultra-performance chromatography column C₁₈, analyzed by using tandem mass spectrometry electrospray ion source under multiple reaction monitoring positive ion mode, and quantified by external standard.

Results  The average recovery in the addition of standard at 5.0 μg/kg, 10 μg/kg and 40 μg/kg was in the range of 65.2%~106.5%, and the relative standard deviation was in the range of 2.4%~14.4%.The linearity was satisfactory with a correlation coefficient of >0.9971 in the concentration of 5 μg/L~100 μg/L. The limit of quantification (LOQ) was 0.24 μg/kg~2.90 μg/kg.

Conclusion  The method was suitable for quantitative analysis and confirmation of antibiotic residues in soil