Toluene Diisocyanate Determination in Water by LC-MS/MS

Objective  To develop a method of directly determining trace toluene diisocyanate in water by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS).

Methods  A 50 μL water sample was directly injected into HPLC, separated by T3 column with isocratic elution of methanol and water, and detected by tandem mass spectrometry in MRM mode. The matrix effect of MS and HPLC was optimized and examined for 10 tap water samples.

Results  The linear range for 6 kinds of estrogen was 0.01 ng/mL~10 ng/mL (r>0.995). The relative standard derivation was less than 5%. The recovery was 97%~100%.

Conclusions  The advantage of this method was sensitive, fast and accurate. The method can be used to directly detect trace amount of toluene diisocyanate in water.