Objectives To establish a RP-HPLC method for the determination of glucosamine hydrochloride to avoid some problems which might cause negative peaks around the peak, bad separation and object peak not retained.
Methods Samples were extracted by ultrasonic wave; the spectrophotometric column was hilic Zorbax Rx-Sil (2.1 mm×150 mm, 5 μm); the mobile phase was 10 mmol/L ammonium acetate-acetonitrile (12:88); the flow rate was 0.7 mL/ min; the detection wavelength was 195 nm; and the column temperature was 30℃.
Results The linear range of glucosamine hydrochloride in this product was 0.2~1.0 mg/mL and the correlation coefficient was 0.9994. The average recovery was 93.8%~96.7%, RSD was 1.02%~2.03%.
Conclusion The content of glucosamine hydrochloride in glucosamine hydrochloride capsule can be determined simply and accurately by this method.
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