PM_2.5 Exposure Discharged from Motor Vehicles on DNA Methylation of EA. Hy926 Cells

Objectives To investigate the proliferative function, DNA methylation and apoptosis of EA.Hy926 cells induced by PM_2.5 discharged from motor vehicles.

Methods EA.Hy926 cells were exposed to PM_2.5 discharged from motor vehicles for 24 h, the proliferative function of cells was determined by MTS method, the DNA methylation of cells was determined by test kits (colorimetric method), and the apoptosis of cells was stained by AnnexinV-FITC/PI and detected by flow cytometry.

Results Compared with the control group, the proliferative function of EA.Hy926 cells was suppressed after 24 h of PM_2.5 exposure in the 200 μg/mL group (P < 0.05); the methylation of EA.Hy926 cells (5-mC%) was significantly decreased after 24 h of exposure to PM_2.5in the 50 and 200 μg/mL group (P < 0.05); the apoptosis of EA.Hy926 cells was increased with the rise of PM_2.5 concentration.

Conclusions The exposure of PM_2.5 discharged from motor vehicles could inhibit the proliferation of EA.Hy926 cells, decrease the level of 5-mC% in EA.Hy926 cells and induce the apoptosis of EA.Hy926 cells, and the decrease of DNA methylation might be the mechanism for inhibited proliferation of EA.Hy926 cells and the apoptosis of EA.Hy926 cells.