Objective To explore the effect of di-2-ethylhexyl phthalate (DEHP) on oxidative stress and apoptosis in GC-2 spd cells.
Methods Immortalized GC-2 spd cells were subcultured and treated with different concentration of DEHP (0, 50, 100 and 200 μmol/L) for 24 h; DEHP was dissolved in 0.1% dimethyl sulfoxide (DMSO). The content of MDA, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in GC-2 spd cells were detected by spectrophotometry, and the apoptosis of GC-2 spd cells was analyzed by flow cytometry.
Results Compared with the control group (0 μmol/L DEHP), the content of MDA was increased with the increase of DEHP, but the difference was not statistically significant (P>0.05). The activity of SOD was decreased significantly in the 200 μmol/L DEHP group (P < 0.05), and the activity of GSH-Px was decreased in the 50 μmol/L and 100 μmol/L DEHP group. The apoptosis of GC-2 spd cells was increased significantly in 100 μmol/L and 200 μmol/L DEHP group (P < 0.05).
Conclusion DEHP might induce apoptosis in GC-2 spd cells by oxidative stress.