Cosmetic Ingredients Sort-term Txicity Assessment

Objective To investigate short-term toxicity of two cosmetic ingredients(No. 1:extraction of semen psoraleae; No. 2:derivative of methylresorcinol).

Methods Dermal irritation was assessed by a 14-day test smearing cosmetic ingredient on the skin of albino rabbit and acute eye irritation was assessed by a modified Draize test. Dermal phototoxicity was tested on the skin of guinea pigs. The irritation and phototoxicity was assessed following the score and grade of related descriptions in Hygienic Standard for Cosmetics. Mutagenicity was tested by chromosome aberration in CHO cells.

Results Cosmetic ingredient No. 1 in a form of original liquid showed a moderate dermal irritation and had a potential of inducing irreversible eye lesion in acute eye irritation test(no flushing or flushing for 30s); using vitamin E in oil as a solvent in a concentration of 20% and 0.5%(v/v) to dilute cosmetic ingredient No. 1 for eye irritation test(no flushing), a mild irritation was observed. For cosmetic ingredient No. 2, both mild dermal irritation and ocular irritation was observed. No positive result was found in dermal phototoxicity test and chromosome aberration test in CHO cells.

Conclusion Cosmetic ingredient No. 1 showed a potential of dermal and ocular irritation; cosmetic ingredient No. 2 showed a mild dermal and ocular irritation, and no positive results in phototoxicity test and chromosome aberration test.