Objectives To establish an optimal condition for separating and measuring 16 kinds of polycyclic aromatic hydrocarbons in PM2.5 by high-performance liquid chromatography(HPLC)with ultraviolet detector.
Methods Polycyclic aromatic hydrocarbons (PAHs) in air were collected by filter membrane and extracted by ultrasonic wave.Using water-acetonitrile as mobile phase, PAHs were efficiently separated by gradient elution and detected by photo-diode-array detector.
Results Sixteen kinds of PAHs were separated efficiently within 35 min and showed good linearity in the range of 0.10~5.00 μg/mL.The relative standard deviations were 1.29%~5.08%, the recoveries were 81.44%~98.02%.
Conclusions PAHs in the air could be analyzed by HPLC because the method was quick, simple, sensitive and reproducible.