DNPH柱前衍生高效液相色谱法测定食品包装材料中的微量甲醛

Determination of Trace Formaldehyde in Food Packaging Materials by DNPH Pre-column Derivation-High Performance Liquid Chromatography

摘要:
目的建立2,4-二硝基苯肼(DNPH)柱前衍生高效液相色谱法测定食品包装材料中微量甲醛的方法。
方法样品浸泡液经DNPH于60℃的水浴中衍生20 min, 用环己烷提取, 液相色谱仪测定。
结果甲醛在0~8.00 μg/mL浓度范围内, 标准曲线线性关系良好, 相关系数r=0.9998, 相对标准偏差为0.9%~2.6%, 加标回收率为91.0%~101.5%。
结论本方法具有灵敏度高、选择性好、精密度和准确度好、简便、快捷等优点。

Abstract:
Objectives To explore the method of DNPH pre-column derivation-high performance liquid chromatography determination of trace formaldehyde in food packaging materials.
Methods After immersed into 2,4-dinitrophenyl hydrazine, the sample was derived 20 min in 60℃ water bath. Trace formaldehyde in food packaging material was determined by liquid chromatography after cyclohexane extraction.
Results The linearity of formaldehyde is in the range of 0~8 μg/mL, correlation coefficient is 0.9998, the relative standard deviation is 0.9%~2.6%, while the recovery rate is 91.0%~101.5%.
Conclusions This method is highly sensitive, selective, preciseand accurate, simple and quick which is suitable for determination of formaldehyde in food packaging materials.