二硝基苯肼柱前衍生-HPLC法测定水中微量甲醛和乙醛

叶坚; 王娴; 余涛

二硝基苯肼柱前衍生-HPLC法测定水中微量甲醛和乙醛

Simultaneous Determination of Trace Formaldehyde and Acetaldehyde in Water by Precolumn Derivation High-performance Liquid Chromatography

摘要

摘要:
目的探讨二硝基苯朋(DNPH)衍生化高效液相色谱(HPTC)法测定水中微量甲醛和乙醛的方法。
方法在60℃的水浴中, 水样中的甲醛和乙醛经DNPH衍生化20 min, 用环己烷提取, 用高效液相色谱仪测定。
结果甲醛、乙醛在0~8.0 μg/mL浓度范围内, 标准曲线线性关系良好, 甲醛和乙醛的相关系数r分别为0.9997和0.9996;甲醛和乙醛的相对标准偏差分别为0.5%~2.4%和1.1%~3.2%;加标回收率分别为97.6%~99.3%和98.4%~101.7%;方法最低检出浓度分别为0.002和0.005 mg/L。甲醛检测结果与国标方法比较无显著性差异(P大于0.05)。
结论本方法不但可同时检测水中微量的甲醛和乙醛, 具有灵敏度高、选择性好、精密度和准确度好等优点, 而且简便、快捷, 适合大批量样品分析。

Abstract:
Objectives To develop a method for the determination of trace formaldehyde and acetaldehyde in water by precolumn derivation high-performance liquid chromatography.
Methods Samples were treated by 2, 4-dinitrophenylhydrazine in 60℃ water bath for derivation for 20 min, and then were extracted with cyclohexane; the contents of formaldehyde and acetaldehyde were finally analyzed by high-performance liquid chromatography quantitatively.
Results The linear ranges for the determination of formaldehyde and acetaldehyde were 0~8.0 μg/mL (r=0.9997) and 0~8.0 μg/mL (r=0.9996), respectively. The relative standard derivations for formaldehyde and acetaldehyde were 0.5%~2.4% and 1.1%~3.2%, respectively. The recoveries were 97.6%~99.3% and 98.4%~101.7% respectively.
Conclusions The method has the advantages of simplicity, repeatability and sensitivity; it can be used for simultaneous analysis of formaldehyde and acetaldehyde in water.

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