900MHz手机频率辐射损伤妊娠SD大鼠肝功能的实验研究

马惠荣; 栗晶晶; 陈景伟; 赵亚楠; 张佳; 于金德; 郭乐乐

900MHz手机频率辐射损伤妊娠SD大鼠肝功能的实验研究

Effect of Radiofrequency Exposure from a 900 MHz Cellular Phone on the Liver of Pregnant Rats

摘要

摘要:
目的研究手机频率辐射对SD妊娠大鼠肝脏结构和功能的影响。
方法将20只SD妊娠大鼠随机分成正常对照组和辐射模型组, 模型组每天接受全身手机频率辐射4 h连续20 d, 产前腹腔麻醉取血和肝组织后处死, 比色法测定血清ALT、AST, HE染色观察肝组织形态学改变和免疫组化检测肝细胞bax、bcl-2蛋白表达。
结果与正常组相比, 手机频率辐射后妊娠大鼠血清ALT增高; HE染色显示肝细胞出现坏死带; 免疫组化结果显示:肝细胞浆bax蛋白表达增强, bcl-2蛋白表达减弱。
结论妊娠期手机频率辐射可损伤SD大鼠肝细胞, 致形态和功能改变, 细胞凋亡增加, 损伤明显。

Abstract:
Objectives To study the radiation of a 900 MHz cellular phone on the liver structure and function of pregnant SD rats.
Methods Twenty pregnant SD rats were selected and randomly divided into a normal group and a model group. The rats in the normal group were not radiated, and the rats in model group were radiated for 4 hours each day in 20 days. The rats were sacrificed by abdominal cavity anesthesia at the end of the experiment. Serum ALT, AST levels were tested by colorimetric method; the histomorphologic change of livers was examined by HE staining and the expression of bax and bcl-2 protein was analyzed by immunohistochemical method.
Results Compared with the normal group, the rats in the model group showed a higher serum ALT, appeared partial degeneration and necrosis of liver cell by HE staining, and a higher expression of bax protein and lower expression of bcl-2 protein analyzed by immunohistochemical method.
Conclusions There was an extremely bad influence of exposure of a 900 MHz cellular phone during gestation on the liver structure and liver function, and an increase of liver apoptosis and induced liver damage.

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