8种增溶剂/溶剂对中国仓鼠卵巢(CHO)细胞活性影响的观察

赵康峰; 董力; 治洪; 张少平; 李毅民

8种增溶剂/溶剂对中国仓鼠卵巢(CHO)细胞活性影响的观察

Influence of 8 Solubilizers/Solvents on the Viability of Chinese Hamster Ovary (CHO) Cells

摘要

摘要:
目的通过观察8种增溶剂/溶剂对中国仓鼠卵巢(CHO)细胞活性的影响，推断其用于CHO细胞染色体畸变试验时的适用浓度。
方法以增溶剂/溶剂作用3 h(+ S9，－ S9) 和24 h(－ S9) 对CHO细胞进行染毒; 刃天青法测定染毒细胞活性; Logit法计算半数抑制浓度IC₅₀和5%抑制浓度IC₅。
结果各增溶剂/溶剂在作用3 h(+ S9，－ S9) 和24 h(－ S9) 后，对CHO细胞的IC₅₀和IC₅影响各异，其中非离子型增溶剂吐温－ 20的IC₅₀小于0. 5 mg / mL(24 h作用组＜0. 05 mg / mL); － S9条件下3 h和24 h的IC₅分别为1. 0和0. 1(丙酮)、1. 1和0. 3(DMSO)、0. 1和＜0. 01 (吐温－ 20)、0. 1和＜0. 01 (吐温－ 60)、1. 1和0. 4 (甲醇)、0. 5和0. 6(95%乙醇)、0. 5和0. 3(乙酸乙酯)和0. 5和1. 5 mg / mL(丙三醇)。
结论 8种增溶剂/溶剂能够不同程度影响CHO细胞的活性，日常检测工作中可参考其IC₅₀和IC₅以确定使用剂量。

Abstract:
Objectives To analyse the effects of 8 solubilizers / solvents on the viability of Chinese Hamster Ovary (CHO) cells to deduce the applicable doses in CHO chromosomal aberration test.
Methods CHO cells were treated with 8 solubilizers / solvents for 3 h(+ S9 － S9) and 24 h (－ S9) respectively. The viability of CHO was detected by resazurin method. Logit method was used to calculate the 50% inhibitory concentration (IC₅₀) and 5% inhibitory concentration (IC₅).
Results The IC₅₀ and IC₅ values of 8 solubilizers / solvents to CHO cell (treated with or without S9 mixture for 3 h, and treated with S9 mixture for 24 h) were variable. The IC₅₀ values of non － ionic solubilizer Tween － 20 were less than 0. 5 mg / mL (especially less than 0. 05 mg / mL in the 24 h treatedt group). IC₅ values in 3 and 24 h treated groups (without S9 mixture) were 1. 0 and 0. 1 (acetone), 1. 1 and 0. 3 (DMSO), 0. 1 and less than 0. 01(Tween － 20), 0. 1 and < 0. 01 (Tween － 60), 1. 1 and 0. 4 (methanol), 0. 5 and 0. 6 (95% ethanol), 0. 5 and 0. 3 (ethyl acetate), and 0. 5 and 1. 5 mg / mL (glycerol) respectively.
Conclusions There were some influences of 8 solubilizers / solvents on the viability of CHO in different degrees. The dosage used in the usual examination may refer to the IC₅₀ and IC₅ of each solubilizer / solvent.

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