高效液相色谱-荧光检测法测定饮用水中双酚A

Determination of Bisphenol A in Drinking Water by High Performance Liquid Chromatography with Fluorescence Detector

摘要:
目的建立生活饮用水中双酚A的液相色谱-荧光检测方法, 为《生活饮用水标准检验方法》(GB/T 5750-2006) 的修订提供参考依据。
方法离心后的水样, 以甲醇/水为流动相, 经C₁₈液相色谱柱分离后, 用液相色谱-荧光检测器测定双酚A含量。
结果双酚A最低定量浓度为0.002 mg/L, 回收率为95%~105%, 相对标准偏差小于2%。
结论本方法无需进行样品富集, 最低检测限满足生活饮用水卫生标准对双酚A含量的测定要求。

Abstract:
Objectives To develop an analytical method for the determination of bisphenol A in drinking water by high performance liquid chromatography with fluorescence detector, and to provide reference basis for revising the "Health Standards for Drinking Water"(GB/T 5750-2006) in the future.
Methods After drinking water samples being centrifuged and separated by C₁₈ column and using methanol/water as mobile phase, the content of bisphenol A was determined by HPLC.
Results The limit of quantification was 0.002 mg/L, the recovery rate was 95%~105%, and the RSD was less than 2%.
Conclusions The results of this method could meet the demands of detecting bisphenol A in drinking water without the operation of sample enrichment.