高效液相色谱-柱后衍生法测定生活饮用水中呋喃丹、甲萘威

卢丽明; 丘福保; 黄诚; 吴惠刚

高效液相色谱-柱后衍生法测定生活饮用水中呋喃丹、甲萘威

Detecting Carbofuran and Carbaryl in Drinking Water by HPLC-post Column Derivatization

摘要

摘要:
目的建立高效液相色谱-柱后衍生同时测定生活饮用水中呋喃丹和甲萘威残留量的方法。
方法样品经二氯甲烷提取, 氮吹浓缩, 用甲醇-水(55:45) 为流动相等度洗脱替代国标法甲醇-水梯度洗脱, 通过柱后衍生反应后, 采用荧光检测器同时测定呋喃丹和甲萘威。
结果在选定的色谱条件下, 以甲醇-水(55:45) 为流动相测定呋喃丹和甲萘威, 方法标准曲线线性关系良好, 其相关系数r>0.999;相对标准偏差2.0%~4.3%(n=6);呋喃丹和甲萘威的平均回收率分别为99.05%和91.56%, 最低检测质量浓度分别为0.4 μg/L和0.2 μg/L。
结论该方法操作简便, 方法精密度、准确度均能满足实验要求, 结果可靠, 适用于生活饮用水中呋喃丹和甲萘威残留量分析。

Abstract:
Objectives To develop a method of detecting carbofuran and carbaryl simultaneously in drinking water by high-performance liquid chromatography (HPLC) with fluorescence detector.
Methods Samples were extracted by dichloromethane, concentrated by N₂ gas, and then separated through a C₁₈ column by using methanol/water (55:45) as mobile phase. After post-column derivatization, the contents of carbofuran and carbaryl were detected by fluorescence detector.
Results The average recoveries of carbofuran and carbaryl were 99.05% and 91.56%, their correlation coefficient was greater than 0.999, the RSD was 2.0%~4.3% (n=6), and the minimum detectable concentration was 0.4 μg/L and 0.2 μg/L, respectively.
Conclusions The advantage of this method was timesaving, and the results were reliable. The sensitivity and accuracy were suitable for the needs of detecting trace amount of carbofuran and carbaryl in drinking water.

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