两种铜制储水容器的抑菌效果评价

张续; 刘锋平; 张倩; 叶必雄; 毕怡文; 宋邦国; 陈远生; 潘力军

两种铜制储水容器的抑菌效果评价

Evaluation on Bacteriostatic Effect of Water Storage Container Made of Copper

摘要

摘要:
目的评价黄铜和红铜两种铜制容器对饮用水中大肠杆菌的生长抑制作用, 为铜制容器的最佳储水方式提供科学参考。
方法分别用两种铜制容器和塑料容器储存不同接种密度大肠杆菌的水样一周, 于0、4、8、24、48、72、96和168 h采集水样。测定水样中总大肠菌群浓度的变化情况, 结合分析两种铜制容器Cu、Zn金属溶出规律, 评价铜制容器对大肠杆菌的抑菌效果。
结果塑料容器无抑菌作用, 而两种铜制容器表现不同程度的抑菌效果。在低接种浓度下, 红铜容器4、8和24h的抑制率为62.5%、83.3%和100%;相同时间下, 黄铜容器的抑制率分别为29.2%、37.5%和100%。在高接种浓度下, 红铜容器4、8和24h的抑菌率为94.8%、99.5%和100%;而黄铜容器的抑菌率分别为0%、0%和98.6%。金属溶出结果显示, 黄铜容器中Zn显著溶出而Cu不溶出, Zn的最高溶出浓度可达1.46 mg/L; 而红铜容器中, Cu显著溶出而Zn不溶出, Cu的最高溶出浓度为0.84 mg/L。
结论在一定条件下, 两种铜制容器对大肠杆菌均具有抑制效应, 且红铜容器的抑制效果优于黄铜容器, 其主要因素可能是溶出金属的种类及溶出质量浓度不同所致。

Abstract:
Objectives To evaluate the bacteriostatic effect of water containers made of brass or red-copper on Escherichia Coli, and to provide scientific reference for the optimal method of storing water in containers.
Methods Drinking water samples inoculated with Escherichia Coli at different densities were stored in copper containers or plastic container for a week.The bacteriostatic effect of copper containers on Escherichia Coli in water samples were evaluated at 4, 8, 24, 48, 72, 96 and 168 h of storage.The Most Probable Number (MPN) value of Escherichia Coli was analyzed, and the dissolution of copper and zinc in brass and red-copper container was also detected.
Results No inhibition effect was observed for plastic container on Escherichia Coli, however, the inhibition effects were observed for both brass and red-copper containers.In the low inoculation group, the inhibition rates at 4, 8 and 24 h for red-copper container were 62.5%, 83.3% and 100% respectively, while those for brass container were 29.2%, 37.5% and 100% respectively.In the high inoculation group, the inhibition rates at 4, 8 and 24 h for red-copper container were 94.8%, 99.5% and 100% respectively, whereas those for brass container were 0%, 0% and 98.6% respectively.The dissolution of zinc (Zn) in brass container was significantly higher, with a peak concentration of 1.46 mg/L, but no copper (Cu) was dissolved in water.However, the dissolution of Cu in red-copper container was significantly higher, with a concentration of 0.84mg/L, whereas no dissolution of Zn was found.
Conclusions There was a bacteriostatic effect of both brass and red-copper water containers on Escherichia Coli, and the bacteriostatic effect of red-copper container was better than brass container.The type and concentration of metals dissolved from different copper containers might be the major factors for bacteriostatic effects.

HTML全文

参考文献(10)

施引文献

资源附件(0)