农药代森锌对斑马鱼胚胎脊索发育的影响

Effect of Pesticide Zineb on Development of Notochord in Zebrafish Embryos

摘要:
目的检测广泛应用的农药代森锌的毒性。
方法运用斑马鱼胚胎进行生物学评价。
结果 0.0025, 0.005, 0.01, 0.02, 0.03, 0.05, 0.1, 和1.0μmol/L代森锌染毒并没有引起斑马鱼明显死亡, 但脊索却发生了明显的弯曲, 弯曲比率分别是1.42%, 10.5%, 5.27%, 26.25%, 44.11%, 66.25%, 100%和100%, 这种特异的弯曲与胶原蛋白无明显关联。
结论实验表明短时间低浓度的代森锌可以致使胚胎畸形, 也表明斑马鱼作为环境污染监测, 是一种非常优良的动物模型。

Abstract:
Objective Monitor the toxicity of widely used pesticide Zineb.
Methods Zebrafish embryos were applied as an animal model for a bioassay.
Results It was showed that 0.0025, 0.005, 0.01, 0.02, 0.03, 0.05, 0.1, and 1.0 μmol/L of zineb could induce 4.2%, 10.5%, 5.27%, 26.25%, 44.11%, 66.25%, 100%, and 100% of notochord defect (curve) respectively in zebrafish embryos, but no significant influence on the mortality of zebrafish embryos.There was no significant association between the specific notochord curve and collagen.
Conclusions This experiment indicated that low level and short-term zineb exposure could also cause a fetal malformation, and the zebrfish embryos might be a very good animal model for environment pollution monitoring.