环境样品中军团菌定量检测方法

Quantitative Detection of Legionella in Environmental Samples

摘要:
目的研究从环境样品水和土壤中分离培养军团菌的定量检测方法。
方法应用阴性样品加标回收实验探讨分离培养条件, 提出方法的回收率、最低检出限与计算模式。
结果加标实验显示:水样品最低检出限为1 cfu/mL, 当加标菌量为10cfu/mL时, 全部样品均可检出加标菌, 回收率为81%~95%;土壤样品选用0.01% Tween 80-PBS作为洗脱液, 最低检出限为100 cfu/g, 当加标菌量为1000 cfu/g时, 全部样品均可检出加标菌, 回收率30%~90%。
结论本研究提出了用于水和土壤等环境样品中的军团菌定量检测方法。

Abstract:
Objectives To develop a quantitative detection method for the isolation and culture of legionella in water and soil.
Methods Using a recovery of standard addition test to explore a detected conditions for quantitative isolation and culture, recovery rate, limit of detection and calculation mode.
Results The limits of detection for water and soil samples were 1 cfu/mL and 10² cfu/g, the recoveries were in the range of 81%-95% and 30%-90%, respectively.
Conclusions A quantitative detection method applied to isolate and culture legionella in water and soil was proposed in this study.