宫内苯并(a)芘暴露对胎儿癌症相关基因表达的影响

李永红; 程义斌; 顾珩; 顾雯; 金银龙

宫内苯并(a)芘暴露对胎儿癌症相关基因表达的影响

Impacts of Prenatal Benzo (a) pyrene Exposure on the Expression of Cancer-related Gene of Fetus

摘要

摘要:
目的初步探讨宫内苯并(a)芘(BaP)暴露对胎儿癌症相关基因表达的影响。
方法用高效液相色谱法检测20份新生儿脐带血血清中BaP的浓度，并按照BaP浓度将样本分成不同暴露组，用实时荧光定量PCR检测脐带血RPL27A(ribosomal protein L27a)、NUDT2(Nudix (nucleoside diphosphate linked moiety X)-type motif 2) 和E2F1(E2F transcription factor 1) 基因的表达，分析不同BaP暴露水平与癌症相关基因表达的关系。
结果 20份脐带血血清BaP平均浓度为0.38 ± 0.31 μg/L。按照血清BaP浓度，将20份样本分成5个不同水平的暴露组。随着BaP暴露水平的增高，RPL27A和NUDT2呈现表达上调的趋势，而E2F1呈现表达下调的趋势。
结论宫内BaP暴露可引起胎儿癌症相关基因表达的改变。

Abstract:
Objectives To explore the impacts of prenatal benzo(a) pyrene (BaP) exposure on the expression of cancer-related gene of fetus.
Methods BaP in cord serum of 20 newborns was detected by using high-performance liquid chromatography (HPLC)/fluorescence, and the mRNA of RPL27A, NUDT2 and E2F1 in cord serum was tested by real-time PCR. The relationship between BaP exposure level and gene expression was analyzed.
Results The average level of BaP in 20 cord serum samples was 0.38 ±0.31 ug/L and the samples were grouped into 5 groups according to BaP levels. With the increase of BaP exposure, the expression of RPL27A and NUDT2 genes showed an up-regulated trend, while E2F1 gene showed a down-regulated trend.
Conclusion Prenatal BaP exposure might induce a change of the expression of cancerrelated genes.

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