Abstract: Objectives To apply the high-performance liquid chromatography-tandem mass spectrometry with quadrupole / linear ion trap instrument in water products for detection and analysis of 4 nitrofurans and related metabolites． Method Considering the interference in true sample，a compound scan by multiple reaction monitoring ( MRM) with information-dependent acquisition ( IDA ) to trigger enhanced product ion ( EPI ) mode is used for identification and quantification of nitrofurans and related metabolites． Results There are good linear correlations( r ＞ 0. 999) between the peak areas and concentrations of the four metabolites of nitrofuran antibiotics in the range of 0. 01 － 5. 0 μg / kg， the limit of detection of AHD、AOZ、SEM、AMOZ are 0. 01 μg / kg、0. 001 μg / kg、0. 01 μg / kg、0. 001 μg / kg and the S / N are more than 10． The average recoveries ranged from 76. 3% to 97. 6% and the relative standard deviations are between 1. 36 to 5. 09% in 0. 01、0. 1 和 0. 5 μg / kg three levels respectively． Conclusions Application of MRMIDA-EPI for compound scan to collect data can achieve the aim of quantifying and qualifying analysis in one sample，effectively exclude the false positive results caused by matrix inference．