高效液相色谱法测定辣椒面中苏丹红检测条件的研究

Conditions for Detection of Sudan Red in Chili Powder with High Performance Liquid Chromatography

摘要:
目的建立辣椒面中苏丹红染料高效液相色谱测定方法。
方法样品用正己烷提取, 经过氧化铝柱浓缩及层析萃取净化处理后, 用反相高效液相色谱在紫外可见光检测器下进行色谱分析。
结果辣椒面样品的苏丹红质量浓度范围在0.01~3.20 mg/kg, 苏丹红Ⅰ, Ⅱ, Ⅲ, Ⅳ各组分线性关系良好, 标准曲线回归方程的相关系数r依次为0.9995、0.9992、0.9991和0.9993;4种组分的方法检出限均为0.01 mg/kg。
结论选取合适的检测波长和梯度洗脱比例, 可以得到满意的实验结果。

Abstract:
Objectives To develop a method for the separation and determination of Sudan Ⅰ, Ⅱ, Ⅲ, Ⅳ in chili powder with high performance liquid chromatography.
Methods After samples being extracted by n-hexane, concentrated by alumina column and purified by chromatographic extraction, analysis was conducted by using reversed-phase high performance liquid chromatography with ultraviolet visible light detector.
Results There were a good linear relationship as the concentration of Sudan Ⅰ, Ⅱ, Ⅲ, Ⅳ in chili samples was in the range of 0.01~3.20 mg/kg, the correlation coefficients were 0.9995, 0.9992, 0.9991 and 0.9993, respectively, and the detection limits for these four components were 0.01 mg/kg.
Conclusions Satisfactory results could be obtained by selecting suitable ratio of gradient elution liquids and detection wavelength.