IL-12对电离辐射损伤小鼠血清学指标的影响

李乐; 唐双阳; 何淑雅; 黄波; 唐艳; 肖方竹; 张炜; 龙望远

IL-12对电离辐射损伤小鼠血清学指标的影响

Effect of IL-12 on the Rehabilitation of Mice Injured by Ionizing Radiation

摘要

摘要:
目的研究白介素-12(IL-12) 对辐射损伤小鼠血清学指标的影响, 探讨其在辐射损伤中的修复作用及机理。
方法 32只实验小鼠分为正常对照组、单纯辐射损伤组、辐射组+空载体组、辐射组+IL-12组。ELISA检测血清干扰素-γ(IFN-γ)和IL-4的含量; 全自动生化分析仪检测其肝肾功能相关生化指标及血糖含量。
结果辐射组+IL-12组血清中IFN-γ含量高于单纯辐射损伤组及辐射组+空载体组, 差异均有统计学意义(P=0.001, P < 0.05);而血清中AST、ALT活性低于单纯辐射损伤组及辐射组+空载体组, 差异均有统计学意义(P值分别为 < 0.001、0.001、0.003和0.016, P < 0.05);血清中IL-4含量、血尿素氮(BUN)、血清肌酐(Scr)浓度及血糖含量差异均无统计学意义(P值分别为0.928、0.964、1.000、1.000、0.680和0.437, P>0.05)。
结论 IL-12能有效提高辐射损伤小鼠的IFN-γ水平, 并促进其血清中转氨酶活性的降低。

Abstract:
Objectives To study the effects of interleukin-12 (IL-12) on some serum indexes of mice in-jured by ionizing radiation; and to investigate its role and mechanism of reducing the injury from radiation.
Methods Thirty two mice were divided into four groups, a normal control group (A), a radiation injured control group (B), a radiation injured and injected with empty vector group (C) and a radiation injured and injected with IL-12 group (D). The contents of interferon-γ(IFN-γ) and IL-4 were detected by ELISA. The biochemical indexes on liver and kidney function and blood sugar were tested by automatic biochemistry analyzer.
Results The content of IFN-γ was higher and the activities of AST and ALT were lower in group D than group B and group C (P < 0.05). The differences of IL-4, blood urea nitrogen (BUN), serum creatinine (Scr) or blood sugar contents a-mong these groups were not significant (P>0.05).
Conclusion IL -12 might be useful in up-regulating the level of serum IFN-γ of the mice injured by ionizing radiation, and down-regulating the activities of serum trans-aminase.

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