Abstract:
Objectives To investigate the neurotoxicity of EOM from greenhouse soil and its mechanism.
Methods EOM samples were extracted by soxhlet method from greenhouse soil, and mice were gavaged at doses of 0, 0. 5, 1. 5, 3. 0 g /mL individually per day for 4 weeks. DMSO was used as a negative control and scopolamine as positive control. Spatial learning and memory were tested with Morris water maze; and the six neuro-biochemical indexes in cortex and hippocampus, including superoxide dismutase (SOD) activity, malondialdehyde (MDA) concentration, nitric oxide concentration, nitric oxide synthase (NOS) activity, acetylcholinesterase (AChE) activity and acetylcholine concentration, were detected after treatment.
Results There were significant differences between groups exposed to high EOM and negative control for place navigation test and spatial probe test; and those for neuro-biochemical indexes except for the activity of SOD.
Conclusion Neurotoxicity could be induced by EOM from greenhouse soil.