温室土壤有机提取物神经毒性研究

徐辉; 任有勋; 闫绍妹; 邢杰; 邵丽军; 邱玉刚; 翟庆峰

温室土壤有机提取物神经毒性研究

Neurotoxicity of Extractable Organic Matter (EOM) from Greenhouse Soil

摘要

摘要:
目的探寻温室土壤中有机提取物(EOM)的神经毒性作用及其可能的作用机制。
方法首先用索氏抽提法提取某温室土壤中的EOM。其次将50只昆明雄性小鼠随机分为5组:阴性对照(DMSO)组、EOM低剂量(0.5 g /mL)组、中剂量(1.5 g /mL)组和高剂量(3.0 g /mL)组，以及阳性对照(东莨菪碱)组，每组10只。对小鼠进行EOM灌胃染毒，每天1次，连续染毒4周。最后进行Morris水迷宫实验，并检测小鼠大脑皮质和海马组织中SOD、MDA、NO、NOS、AChE和ACh等神经生化指标。
结果 EOM高、中剂量组定位导航和空间探索指标皆较溶剂对照组有统计学差异。除SOD活性外，EOM高剂量组大脑皮质和海马中以上各神经生化指标皆较溶剂对照组具有统计学差异。
结论土壤中EOM可能是通过诱使NO /NOS和ACh含量降低、脂质氧化性损伤等神经生化指标的变化，来影响小鼠的学习记忆能力。

Abstract:
Objectives To investigate the neurotoxicity of EOM from greenhouse soil and its mechanism.
Methods EOM samples were extracted by soxhlet method from greenhouse soil, and mice were gavaged at doses of 0, 0. 5, 1. 5, 3. 0 g /mL individually per day for 4 weeks. DMSO was used as a negative control and scopolamine as positive control. Spatial learning and memory were tested with Morris water maze; and the six neuro-biochemical indexes in cortex and hippocampus, including superoxide dismutase (SOD) activity, malondialdehyde (MDA) concentration, nitric oxide concentration, nitric oxide synthase (NOS) activity, acetylcholinesterase (AChE) activity and acetylcholine concentration, were detected after treatment.
Results There were significant differences between groups exposed to high EOM and negative control for place navigation test and spatial probe test; and those for neuro-biochemical indexes except for the activity of SOD.
Conclusion Neurotoxicity could be induced by EOM from greenhouse soil.

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