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    姜瑞雪, 万运方, 刘雁云, 胡霞, 牛志尊, 黄密, 马作峰. PM2.5对Wistar大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响[J]. 环境卫生学杂志, 2014, 4(6): 514-517.
    引用本文: 姜瑞雪, 万运方, 刘雁云, 胡霞, 牛志尊, 黄密, 马作峰. PM2.5对Wistar大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响[J]. 环境卫生学杂志, 2014, 4(6): 514-517.
    shu
    Jiang Ruixue, Wan Yunfang, Liu Yanyun, Hu Xia, Niu Zhizun, Huang Mi, Ma Zuofeng. Effects of PM2.5 on HMGB1, TNF-α and IL-6 Expression in Wistar Rats Lung[J]. Journal of Environmental Hygiene, 2014, 4(6): 514-517.
    Citation: Jiang Ruixue, Wan Yunfang, Liu Yanyun, Hu Xia, Niu Zhizun, Huang Mi, Ma Zuofeng. Effects of PM2.5 on HMGB1, TNF-α and IL-6 Expression in Wistar Rats Lung[J]. Journal of Environmental Hygiene, 2014, 4(6): 514-517.
    shu

    PM2.5对Wistar大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响

    Effects of PM2.5 on HMGB1, TNF-α and IL-6 Expression in Wistar Rats Lung

      摘要
    • 摘要:
      目的 观察气管滴注不同浓度大气细颗粒物(PM2.5)混悬液对Wistar大鼠肺组织中高迁移率蛋白(HMGB1)、肿瘤坏死因子-α(TNF-α)和白细胞介素6(IL-6) 表达的影响, 为进一步探求中医防治雾霾伤肺的有效方药提供客观依据。
      方法 采用便携式大气细颗粒物PM2.5采样器收集武汉工业区大气中的PM2.5, 染毒前用生理盐水配制成所需浓度的PM2.5混悬液。将40只健康SPF级雄性Wistar大鼠随机分为空白组、生理盐水组、PM2.5低、中、高浓度组(PM2.5混悬液的浓度分别为1、3、10 mg/mL)。检测大鼠肺组织中HMGB1、TNF-α、IL-6的表达水平。
      结果 PM2.5低浓度组、PM2.5中浓度组、PM2.5高浓度组的HMGB1、TNF-α、IL-6表达水平均高于空白组和生理盐水组, 差异有统计学意义(P＜0.01)。进一步经LST-t检验, 在HMGB1、TNF-α的表达方面, PM2.5低、中浓度组组间比较, 差异无统计学意义(P＜0.05), 两组与PM2.5高浓度组比较, 差异均具有统计学意义(P＜0.05);在IL-6的表达方面, PM2.5低浓度组与PM2.5中、高浓度组比较, 差异有统计学意义(P＜0.05);PM2.5中浓度组与PM2.5高浓度组比较, 差异无统计学意义(P＜0.05)。
      结论 随着PM2.5浓度的增加, 染毒组大鼠肺组织中HMGB1、TNF-α和IL-6的表达水平均显著增高, 肺组织中HMGB1、TNF-α和IL-6的表达有望作为雾霾致大鼠肺损伤的生物学指标。

       

      Abstract:
      Objectives To investigate the effects of PM2.5 on the expression of HMGB1, TNF-a and IL-6 in the lung of Wistar rats to provide an objective basis for effective prevention and treatment of lung injury caused by PM2.5 in haze.
      Methods The atmospheric PM2.5 in Wuhan industrial zone was collected by a portable PM2.5 sampler. PM2.5 suspensions were prepared by saline. Forty healthy male SPF Wistar rats were randomly divided into five groups:a control group, a saline instillation group and three PM2.5 exposure groups treated by tracheal instillation of low, medium and high levels of PM2.5 suspensions (1, 3 and 10mg/mL respectively). The expression of HMGB1, TNF-α and IL-6 in the lung of Wistar rats were observed and analyzed.
      Results Compared with the control group and saline instillation group, the expression of HMGB1, TNF-and IL-6 in PM2.5 exposure groups was higher (P < 0.01). In the LST-t experiment, the expression of HMGB1 and TNF-α between the low and medium PM2.5 exposure group were not significantly different (P < 0.05), whereas they were lower than the high PM2.5 exposure group (P < 0.05). The expression of IL-6 in the low PM2.5 exposure group was significant lower than the high and medium PM2.5 exposure group (P < 0.05), but no statistically significant difference between the high and medium PM2.5 exposure group (P < 0.05).
      Conclusions With increasn of PM2.5 in suspension, the expression of HMGB1, TNF-α and IL-6 in the lung of rats were signif icantly increased. The expression of HMGB1, TNF-α and IL-6 in lung tissue might be used as biological indicators for lung damage caused by PM2.5.

       

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