4种甲型H1N1流感病毒试剂盒检测效果对比分析

巩飚; 曹晓; 胡淮洁; 许姣; 李柯

4种甲型H1N1流感病毒试剂盒检测效果对比分析

Comparison and Analysis of Detection Effects of Four Kinds of Diagnostic Kits for Influenza A H1N1 Virus

摘要

摘要:
目的对比分析4种甲型H1N1流感病毒检测试剂盒的实际检测效果, 为试剂盒的开发和改良提供参考。
方法采用Real-time RT-PCR法检测甲型H1N1流感病毒, 利用SPASS 13.0软件进行数据分析。
结果不同检测试剂盒其检出阳性率不同, 其中试剂盒A和试剂盒C的检出阳性率均为100%, 试剂盒B为95%, 而试剂盒D检出阳性率仅为80%。以单个指标为研究对象, 经方差分析及SNK检验, 显示4种检测试剂盒对每个指标的检测效果都存在显著差异, 其扩增效率有明显不同。
结论试剂盒A、B和C均可检测甲型H1N1流感病毒而试剂盒C灵敏性稍高更适宜用于初筛, 试剂盒D技术上需要进一步改进。

Abstract:
Objectives To provide reference for developing and improving diagnostic kits by comparison and analysis of the detection effects of four kinds of diagnostic kits in practice.
Methods Real-time RT-PCR was employed to detect Influenza A H1N1 virus and SPASS 13.0 was applied to analyze data.
Results The positive rates of different diagnostic kits were different. Among them, the positive rates of kit A, kit C, and kit B were respectively 100%, 100% and 95%, while the kit D was merely 80%. Furthermore, tested by analysis of variance and SNK Multi-Analysis with singular index, the results showed that there were significant differences among all of the four kinds of diagnostic kits for each indicator and the amplification efficiency of which was obviously different.
Conclusions Kit A, B and C can be used for detection of Influenza A H1N1 virus. However, kit C was slightly higher sensitive and more suitable for preliminary screening. Furthermore, kit D needs further improving in technic.

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