纳米四氧化三铁颗粒对小鼠骨髓细胞DNA损伤作用
DNA Damage of Bone Marrow Cells in Mice Induced by Nano-Fe3O4 Particles
-
摘要:目的 观察纳米四氧化三铁颗粒(Nano-Fe3O4)对小鼠骨髓细胞DNA损伤作用。方法 将40只成年清洁级ICR小鼠, 雌雄各随机分为4组, 分别为溶剂对照(高纯水)组和低剂量(2.5 mg/kg, 1/64 LD50)、中剂量(10 mg/kg, 1/16 LD50)和高剂量(40 mg/kg, 1/4 LD50) Nano-Fe3O4染毒组, 每组5只。采用一次性尾静脉注射方式进行染毒, 注射量为10 mL/kg, 注射速度约为0.02 mL/s。染毒后3 d, 用单细胞凝胶电泳法检测其对小鼠骨髓细胞DNA损伤。结果 染毒3 d后, 雌性小鼠骨髓细胞DNA 0级损伤各剂量组明显低于对照组(P＜0.05), 并且随着剂量的增加无损伤的比例逐渐减少, Ⅰ级损伤各剂量组明显高于对照组(P＜0.05), 且随着剂量升高损伤程度逐渐增强, 仅高剂量组出现≥Ⅱ级损伤。雄性小鼠骨髓细胞DNA 0级损伤各剂量组明显低于对照组(P＜0.05), 并且随着剂量的升高无损伤的比例逐渐降低, Ⅰ级损伤中各剂量组明显高于对照组(P＜0.05), 且随着剂量的升高损伤程度逐渐增强, 仅高剂量组出现≥Ⅱ级损伤。结论 在本实验条件下, 一次性尾静脉注射Nano-Fe3O4颗粒3 d后, 可引起小鼠骨髓细胞的DNA损伤。Abstract:Objectives To observe the effect of Nano-Fe3O4 on the DNA damage of bone marrow cell in mice.Methods Forty clean grade ICR mice were divided into four groups by random with equal numbers of male and female in each group.They were high-dosage-treated group (40 mg/kg, 1/4 LD50), middle-dosage-treated group (10 mg/kg, 1/16 LD50), low-dosage-treated group (2.5 mg/kg, 1/64 LD50), and pure water negative control group.Nano-Fe3O4 in a volume of 10 mL/kg and with a speed of 0.02 mL/s was injected once through tail vein.The DNA damage of bone marrow cells was detected three days after injection of Nano-Fe3O4 by using single cell gel electrophoresis.Results The percentage of DNA damage at grade 0 of all Nano-Fe3O4 treated groups were obviously lower than those in the control group(P < 0.05).There was a tendency of increasing the dosage of Nano-Fe3O4 with an obviously higher grade of DNA damage (P < 0.05).And the DNA damage at grade ≥Ⅱ was appeared only in the high-dosage-treated group.Conclusion Nano-Fe3O4 could cause DNA damage of marrow cells in mice injected intravenously after three days.
下载: