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    侯敏, 丁珵, 邓富昌, 田哲, 丁培, 王友斌, 徐凯强, 姚巧, 唐宋, 姚孝元. 城市污水中诺如病毒两种富集浓缩方法的比较研究[J]. 环境卫生学杂志, 2023, 13(5): 357-362. DOI: 10.13421/j.cnki.hjwsxzz.2023.05.009
    引用本文: 侯敏, 丁珵, 邓富昌, 田哲, 丁培, 王友斌, 徐凯强, 姚巧, 唐宋, 姚孝元. 城市污水中诺如病毒两种富集浓缩方法的比较研究[J]. 环境卫生学杂志, 2023, 13(5): 357-362. DOI: 10.13421/j.cnki.hjwsxzz.2023.05.009
    shu
    HOU Min, DING Cheng, DENG Fu-chang, TIAN Zhe, DING Pei, WANG You-bin, XU Kai-qiang, YAO Qiao, TANG Song, YAO Xiao-yuan. Two enrichment methods for norovirus in urban sewage: a comparative study[J]. Journal of Environmental Hygiene, 2023, 13(5): 357-362. DOI: 10.13421/j.cnki.hjwsxzz.2023.05.009
    Citation: HOU Min, DING Cheng, DENG Fu-chang, TIAN Zhe, DING Pei, WANG You-bin, XU Kai-qiang, YAO Qiao, TANG Song, YAO Xiao-yuan. Two enrichment methods for norovirus in urban sewage: a comparative study[J]. Journal of Environmental Hygiene, 2023, 13(5): 357-362. DOI: 10.13421/j.cnki.hjwsxzz.2023.05.009
    shu

    城市污水中诺如病毒两种富集浓缩方法的比较研究

    Two enrichment methods for norovirus in urban sewage: a comparative study

      摘要
    • 摘要:
      目的 比较聚乙二醇沉淀法和阴离子膜吸附—超声振荡法富集浓缩污水中诺如病毒(norovirus, NoV)的回收率, 选择更适用于城市污水中诺如病毒监测工作的富集浓缩方法。
      方法 将诺如病毒稀释三个梯度分别加入到城市生活污水(100 mL)中, 采用聚乙二醇沉淀法和阴离子膜吸附—超声振荡法进行富集浓缩, 采用实时荧光反转录聚合酶链式反应(RT-PCR)检测富集浓缩后GI型和GⅡ型病毒浓度, 并进一步计算回收率。
      结果 结果表明: ①当NoV GI型浓度为101.4 copies/μL时, 阴离子膜吸附—超声振荡法回收率高于聚乙二醇沉淀法(P < 0.05)。②当NoV G Ⅱ型浓度为102.6copies/μL时, 阴离子膜吸附—超声振荡法回收率高于聚乙二醇沉淀法(P < 0.05)。
      结论 综合考虑富集浓缩效率等相关因素, 阴离子膜吸附—超声振荡法更适合小体积污水中诺如病毒的富集浓缩。

       

      Abstract:
      Objective To investigate a more suitable enrichment method for norovirus (NoV) monitoring in urban sewage by comparing the recovery rate of NoV in sewage by polyethylene glycol (PEG) precipitation versus electronegative membrane adsorption-ultrasonic oscillation.
      Methods NoV was diluted in three gradients and added into urban domestic sewage (100 mL), then concentrated by the two method of PEG precipitation and electronegative membrane adsorption-ultrasonic oscillation. The concentrations of GI and GⅡ virus after enrichment were detected by real-time fluorescent reverse transcription polymerase chain reaction (RT-PCR), and then the recovery rate was calculated.
      Results When the concentration of NoV GI was 101.4 copies/μL, electronegative membrane adsorption-ultrasonic oscillation had a higher recovery rate than PEG precipitation (P < 0.05). When the concentration of NoV GII was 102.6 copies/μL, electronegative membrane adsorption-ultrasonic oscillation had a higher recovery rate than PEG precipitation (P < 0.05).
      Conclusion Considering concentration efficiency and other related factors, the method of electronegative membrane adsorption-ultrasonic oscillation is more suitable for the enrichment of NoV in a small volume of sewage.

       

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