Objective To establish a method for simultaneous determination of six isothiazolinone bactericides in wet wipes by GC with flame photometric detector (FPD).

Methods The samples were extracted by ethanol ultrasonic oscillation for 30 min, dehydrated by anhydrous sodium sulfate, separated by a DB-624 chromatographic column, determined by GC with FPD detector, and then quantified by the external standard method.

Results Under the optimized experimental conditions, the concentration of six isothiazolinone bactericides showed a good linear relationship with peak area after quadratic curve fitting within the range of 1-60 μg/mL, with a correlation coefficient above 0.9995. The detection limit was 0.24-0.53 μg/mL, and the quantification limit was 0.80-1.77 μg/mL. The average recovery rate was 92.3%-108.7%, with a relative standard deviation of 4.6%-10.7%.

Conclusion This method is simple and convenient with good repeatability and can be used for the determination of six isothiazolinone bactericides in wet wipes.