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    康惠文, 宋倩, 陈晨, 石聪, 李清钊, 王梦迪, 杨舒越, 蒋守芳. 大气PM2.5对大鼠海马组织脑源性神经营养因子及其受体TrkB蛋白表达的影响[J]. 环境卫生学杂志, 2022, 12(3): 166-169, 176. DOI: 10.13421/j.cnki.hjwsxzz.2022.03.002
    引用本文: 康惠文, 宋倩, 陈晨, 石聪, 李清钊, 王梦迪, 杨舒越, 蒋守芳. 大气PM2.5对大鼠海马组织脑源性神经营养因子及其受体TrkB蛋白表达的影响[J]. 环境卫生学杂志, 2022, 12(3): 166-169, 176. DOI: 10.13421/j.cnki.hjwsxzz.2022.03.002
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    KANG Hui-wen, SONG Qian, CHEN Chen, SHI Cong, LI Qing-zhao, WANG Meng-di, YANG Shu-yue, JIANG Shou-fang. Effect of atmospheric PM2.5 on the protein expression of brain-derived neurotrophic factor and its receptor TrkB in the hippocampus tissue of rats[J]. Journal of Environmental Hygiene, 2022, 12(3): 166-169, 176. DOI: 10.13421/j.cnki.hjwsxzz.2022.03.002
    Citation: KANG Hui-wen, SONG Qian, CHEN Chen, SHI Cong, LI Qing-zhao, WANG Meng-di, YANG Shu-yue, JIANG Shou-fang. Effect of atmospheric PM2.5 on the protein expression of brain-derived neurotrophic factor and its receptor TrkB in the hippocampus tissue of rats[J]. Journal of Environmental Hygiene, 2022, 12(3): 166-169, 176. DOI: 10.13421/j.cnki.hjwsxzz.2022.03.002
    shu

    大气PM2.5对大鼠海马组织脑源性神经营养因子及其受体TrkB蛋白表达的影响

    Effect of atmospheric PM2.5 on the protein expression of brain-derived neurotrophic factor and its receptor TrkB in the hippocampus tissue of rats

      摘要
    • 摘要:
      目的 探讨大气PM2.5染毒对大鼠海马组织中脑源性神经营养因子(BDNF)及其受体TrkB相关蛋白表达的影响。
      方法 采集大气PM2.5，制备混悬液。将48只SPF级雄性SD大鼠随机分为对照组和暴露组，每组8只，染毒组大鼠按照20 mg/kg气管滴注PM2.5混悬液，对照组大鼠气管滴注等量生理盐水，每周1次，分别染毒1个月、3个月和6个月。采用免疫组化法测定大鼠海马组织中PKA蛋白表达，蛋白免疫印迹法测定大鼠海马组织中BDNF、TrkB、AKT和CREB蛋白表达水平。
      结果 与相应对照组比较，3个月、6个月染毒组大鼠海马组织中PKA阳性细胞减少，BDNF、TrkB、AKT和CREB蛋白表达水平均明显降低，差异均有统计学意义(P < 0.05)。
      结论 大气PM2.5染毒可降低大鼠海马组织中BDNF、TrkB、AKT、CREB和PKA蛋白表达水平。

       

      Abstract:
      Objective To investigate the effect of atmospheric fine particulate matter (PM2.5) exposure on the protein expression of brain-derived neurotrophic factor (BDNF) and its related proteins of tyrosine receptor kinase B (TrkB) in the hippocampus tissue of rats.
      Methods PM2.5 was collected to prepare a suspension. Forty-eight specific pathogen-free male Sprague-Dawley rats were randomly divided into control groups and exposure groups, with 8 rats in each group. Rats in the exposure groups were treated with 20 mg/kg PM2.5 suspension via intratracheal instillation, and rats in the control groups were treated with the same amount of normal saline. The treatments were performed once a week for 1 month, 3 months, and 6 months, respectively. The protein expression of PKA in the hippocampal tissue of the rats was determined by immunohistochemistry, and the protein expression levels of BDNF, TrkB, AKT, and CREB in the hippocampal tissue of the rats were determined by Western blot.
      Results Compared with the corresponding control groups, the 3-and 6-month exposure groups had significantly decreased PKA positive cells in the hippocampus tissue of the rats and significantly decreased protein expression levels of BDNF, TrkB, AKT, and CREB (P < 0.05).
      Conclusion Exposure to atmospheric PM2.5 can reduce the protein expression levels of BDNF, TrkB, AKT, CREB, and PKA in the hippocampus tissue of rats.

       

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