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    孙钰, 程斌, 张家泰, 董淑英. DEHP诱导人肝癌HuH-7细胞增殖迁移及二甲双胍对其抑制作用[J]. 环境卫生学杂志, 2021, 11(5): 403-407, 414. DOI: 10.13421/j.cnki.hjwsxzz.2021.05.002
    引用本文: 孙钰, 程斌, 张家泰, 董淑英. DEHP诱导人肝癌HuH-7细胞增殖迁移及二甲双胍对其抑制作用[J]. 环境卫生学杂志, 2021, 11(5): 403-407, 414. DOI: 10.13421/j.cnki.hjwsxzz.2021.05.002
    shu
    SUN Yu, CHENG Bin, ZHANG Jiatai, DONG Shuying. Human Hepatoma HuH-7 Cells Proliferation and Migration Induced by DEHP and Its Inhibition by Metformin[J]. Journal of Environmental Hygiene, 2021, 11(5): 403-407, 414. DOI: 10.13421/j.cnki.hjwsxzz.2021.05.002
    Citation: SUN Yu, CHENG Bin, ZHANG Jiatai, DONG Shuying. Human Hepatoma HuH-7 Cells Proliferation and Migration Induced by DEHP and Its Inhibition by Metformin[J]. Journal of Environmental Hygiene, 2021, 11(5): 403-407, 414. DOI: 10.13421/j.cnki.hjwsxzz.2021.05.002
    shu

    DEHP诱导人肝癌HuH-7细胞增殖迁移及二甲双胍对其抑制作用

    Human Hepatoma HuH-7 Cells Proliferation and Migration Induced by DEHP and Its Inhibition by Metformin

      摘要
    • 摘要:
      目的 研究邻苯二甲酸二(2-乙基己基)酯(DEHP)诱导人肝癌细胞HuH-7增殖迁移作用及二甲双胍对其抑制作用。
      方法 镜下观察细胞形态变化, MTT细胞活性测定法检测DEHP诱导的增殖作用, 二甲双胍、PI3K抑制剂(LY294002)的抑制作用。通过划痕—修复实验和Transwell迁移实验观察DEHP诱导HuH-7细胞的迁移作用及二甲双胍、LY294002的抑制作用。
      结果 浓度为40和80 μg / mL的DEHP可以显著促进HuH-7细胞增殖, 与对照组相比差异具有统计学意义(P < 0.05), 该增殖作用可被浓度为10 mg / mL的二甲双胍、浓度为50 μmol / L的LY294002抑制(P < 0.05)。划痕—修复实验和Transwell实验证明DEHP可以诱导HuH-7细胞迁移(P < 0.05), 该作用可被二甲双胍、LY294002抑制(P < 0.05)。
      结论 DEHP可以促进人肝癌细胞HuH-7增殖与迁移, 二甲双胍可以抑制该增殖迁移作用, 从而起到保护作用。

       

      Abstract:
      Objective To explore the role of bis(2-ethylhexyl) phthalate (DEHP) in inducing the proliferation and migration of human hepatoma HuH-7 cells and metformin inhibitory effect.
      Methods Cell morphology-based observation and MTT assay (for assessing cellular activity) were used to determine DEHP-induced proliferation and the inhibitory effect of metformin and phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002). The wound healing assay and Transwell migration assay were used to assess DEHP-induced migration of HuH-7 cells and the inhibitory effect of metformin and LY294002.
      Results DEHP at 40 and 80 μg/mL significantly promoted the proliferation of HuH-7 cells as compared with that in control group (P < 0.05); and the proliferation was inhibited by metformin at 10 mg/mL and LY294002 at 50 μmol/L (P < 0.05). The wound healing assay and Transwell migration assay proved that DEHP induced HuH-7 cell migration (P < 0.05), which was inhibited by metformin and LY294002 (P < 0.05).
      Conclusion DEHP can promote the proliferation and migration of human hepatoma HuH-7 cells; metformin can inhibit the proliferation and migration, and therefore playsaprotective role.

       

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