长链非编码RNA-TC在苯致细胞凋亡中的作用

任婧; 郭肖利; 张微; 王婧宇; 高艾

doi:10.13421/j.cnki.hjwsxzz.2021.02.007

长链非编码RNA-TC在苯致细胞凋亡中的作用

The Role of Long Non-Coding RNA-TC in Benzene-induced Apoptosis

摘要

摘要:
目的探讨长链非编码RNA-TC（Lnc-TC）在苯毒性中的作用及机制。
方法运用不同剂量苯代谢物1，4苯醌（1，4-benzoquinone，1，4-BQ）（0、10、20和40 μM）染毒人B淋巴细胞（AHH-1）24 h后，MTT法检测细胞活力；透射电镜和共聚焦显微镜检测细胞凋亡的情况；qRT-PCR检测Lnc-TC以及凋亡执行基因caspase-3的表达。体外利用慢病毒构建Lnc-TC干扰模型，探讨干扰Lnc-TC对1，4-BQ诱导的细胞凋亡的影响。
结果实验结果显示，1，4-BQ可剂量依赖性导致AHH-1细胞活力降低，诱导细胞形态异常伴随凋亡小体出现。共聚焦显微镜下显示1，4-BQ诱导凋亡细胞数量增多，伴随Lnc-TC和凋亡执行基因caspase-3表达显著上调，而干扰Lnc-TC后，发现可以缓解1，4-BQ诱导的细胞凋亡，并且逆转caspase-3表达。
结论 Lnc-TC通过调控凋亡执行基因caspase-3的表达诱导细胞凋亡导致苯的血液毒性。

Abstract:
Objective To investigate the role and mechanism of long non-coding RNA-TC(Lnc-TC)in benzene-induced hematotoxicity toxicity.
Methods Different doses of benzene metabolite, 1, 4-benzoquinone (1, 4-BQ) were used in vitro to treat human B lymphocytes (AHH-1) for 24 h. Cell viability was detected by MTT method. Transmission electron microscope and immunofluorescence method were used to detect cell apoptosis, and qRT-PCR was used to detect the expression of Lnc-TC and the apoptosis-related gene caspase-3. The Lnc-TC interference model was constructed by lentivirus in vitro to explore the effect on cell apoptosis.
Results The results of cell experiments showed that 1, 4-BQ reduced the viability of AHH-1 cells in a dose-dependent manner, leading to abnormal cell morphology, and the appearance of apoptotic bodies was accompanied by the up-regulation of the expression of caspase-3, the apoptotic executive gene. At the same time, 1, 4-BQ induced a significant up-regulation of the Lnc-TC expression. After interfering with Lnc-TC, it was found that interfering with Lnc-TC alleviated cell apoptosis and reversed the up-regulation of caspase-3 expression caused by 1, 4-BQ.
Conclusion Lnc-TC induces cell apoptosis and leads to benzene hemotoxicity by regulating the expression of the apoptosis executive gene caspase-3.

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