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    阿依谢姆古丽·阿力马斯, 尔西丁·买买提, 鲁英, 赵婷婷. 孕产妇尿中1-羟基芘水平与孕产妇及新生儿hOGG1和XRCC1基因多态性的研究[J]. 环境卫生学杂志, 2019, 9(5): 425-429. DOI: 10.13421/j.cnki.hjwsxzz.2019.05.003
    引用本文: 阿依谢姆古丽·阿力马斯, 尔西丁·买买提, 鲁英, 赵婷婷. 孕产妇尿中1-羟基芘水平与孕产妇及新生儿hOGG1和XRCC1基因多态性的研究[J]. 环境卫生学杂志, 2019, 9(5): 425-429. DOI: 10.13421/j.cnki.hjwsxzz.2019.05.003
    shu
    ALIMASI Ayixiemuguli, MAIMAITI Erxiding, LU Ying, ZHAO Tingting. Study on the Level of Maternal Urinary 1-Hydroxypyrene and the Polymorphism of Maternal and Neonatal hOGG1 and XRCC1 Genes[J]. Journal of Environmental Hygiene, 2019, 9(5): 425-429. DOI: 10.13421/j.cnki.hjwsxzz.2019.05.003
    Citation: ALIMASI Ayixiemuguli, MAIMAITI Erxiding, LU Ying, ZHAO Tingting. Study on the Level of Maternal Urinary 1-Hydroxypyrene and the Polymorphism of Maternal and Neonatal hOGG1 and XRCC1 Genes[J]. Journal of Environmental Hygiene, 2019, 9(5): 425-429. DOI: 10.13421/j.cnki.hjwsxzz.2019.05.003
    shu

    孕产妇尿中1-羟基芘水平与孕产妇及新生儿hOGG1和XRCC1基因多态性的研究

    Study on the Level of Maternal Urinary 1-Hydroxypyrene and the Polymorphism of Maternal and Neonatal hOGG1 and XRCC1 Genes

      摘要
    • 摘要:
      目的 探讨乌鲁木齐市孕产妇多环芳烃（polyeyclic aromatic hydrocarbons，PAHs）暴露与孕产妇及新生儿hOGG1和XRCC1基因多态性之间的关系。
      方法 采集乌鲁木齐市某家医院115位孕产妇尿液、静脉血及新生儿脐带血，采用聚合酶链反应—限制性片段长度多态性分析（PCR-RFLP）孕产妇与新生儿的hOGG1和XRCC1的基因位点多态性，采用酶水解—液相色谱—质谱联用仪检测孕产妇尿中PAHs内暴露标志物1-羟基芘（1-OH-P）。
      结果 孕产妇尿中1-OH-P浓度为0.340（0.208~0.555）μg/mmol Cr。孕产妇hOGG1326Ser/Cys基因型不同，体内1-OH-P的浓度也不同（P < 0.05）。携带不同的XRCC1399Arg/Gln基因型新生儿，其母亲体内1-OH-P的浓度存在统计学差异（P < 0.05）。携带不同XRCC1399Arg/Gln基因型的孕产妇，其体内的1-OH-P的浓度差别无统计学意义（P>0.05）。携带不同的hOGG1326Ser/Cys基因型的新生儿，其母亲的体内1-OH-P的浓度无统计学差异（P>0.05）。
      结论 与国内相关研究相比，乌鲁木齐市孕产妇体内的PAHs处于高暴露水平。携带hOGG1326Ser/Cys基因的孕产妇机体在PAHs高暴露环境中DNA损伤更加敏感。

       

      Abstract:
      Objectives To investigate the relationship between maternal polycyclic aromatic hydrocarbon(PAHs) exposure in Urumqi and the polymorphisms of maternal and neonatal hOGG1 and XRCC1 genes.
      Methods A total of 115 maternal urine, venous blood and neonatal cord blood were collected from a hospital in Urumqi, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for gene locus polymorphism of maternal and neonatal hOGG1 and XRCC1. The use of enzymatic hydrolysis-liquid chromatography-mass spectrometry to detect internal exposure marker of PAHs, 1-OH-P, in the maternal urine.
      Results The median level of 1-OH-Pconcentration in maternal urine was 0.340 (0.208~0.555) μg/mmol Cr. The maternal hOGG1326Ser/Cys genotype was different, and the concentration of 1-OH-Pin the body was also different (P < 0.05). There was a statistically significant difference in the concentrations of 1-OH-P in the mothers with neonates carrying different XRCC1399Arg/Gln genotypes(P < 0.05). There was no significant difference in the concentration of 1-OH-P between the parturient carrying different XRCC1399Arg/Gln genotypes (P>0.05). There was no significant difference in the concentration of 1-OH-Pin the mothers with neotates carrying different hOGG1326Ser/Cys genotypes (P>0.05).
      Conclusions Compared with domestic related research, PAHs in parturient in Urumqi were at a high the exposure levels. Maternal bodies carrying the hOGG1326Ser/Cys gene are more susceptible to DNA damage in the high exposure environment of PAHs.

       

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