Abstract:
Objectives To comparing the ability of LLNA:BrdU-ELISA assay and skin irritation assay to assesse the irritation of hair dyes.
Methods Irritation of 15 hair dyes was tested according to OECD442 (B) and OECD404 standard methods.
Results In LLNA:BrdU-ELISA method, 5 kinds of hair dyes had irritation. In skin irritation assay, 3 kinds of hair dyes had light irritation and 2 kinds hair dyes had moderate irritation.
Conclusions The ability of LLNA:BrdU-ELISA method to evaluate sensitizing of hair dyes was equal to that of skin irritation assay.