纸片法检测水中菌落总数的应用与评价

张雅婕; 张永; 田佩瑶; 张桂斌; 史燕萍

doi:10.13421/j.cnki.hjwsxzz.2019.04.015

纸片法检测水中菌落总数的应用与评价

Application and Evaluation of Detection for Total Number of Colonies in Water by Paper Method

摘要

摘要:
目的应用纸片法检测水中菌落总数并对其进行应用可行性分析。
方法用纸片法对40份不同类型水样进行重复性试验，用纸片法和国标法同时检测不同类型水样（共160份）的菌落总数并对检测结果进行比较。
结果重复性试验纸片法的变异系数在1.16%~4.97%；纸片法菌落总数的检测结果与国标法检测结果呈正相关，r>0.824，P < 0.001；但两种方法的检测数据结果差异有统计学意义（P < 0.05）。
结论纸片法检测结果与国标法有相关性，且具有操作简单、适合现场检测等特点，可作为水质微生物污染的趋势监测、污染事故和应急卫生保障等菌落总数的筛查方法，但纸片法仍然不能代替国家标准检验方法。

Abstract:
Objectives To analyze the feasibility and application of detection for total number of colonies in water by paper method.
Methods The repeatability test of 40 different water samples was carried out by paper method. Then paper method and national standard method were simultaneously used to detect the total number of colonies with different types of water samples (n=160), and the result were compared.
Results In the repeatability test, the coefficient of variation of paper method was 1.16%~4.97%. In the comparison experiment, resuls of total number of colonies test with paper method and national standard method were positively correlated (r>0.824, P < 0.001), and there were significant differences in the test result of paper method and national standard method (P < 0.05).
Conclusions Paper method can be used as a trend monitoring of total colonies in water quality or screening method of total number colonies in pollution accidents and emergency hygienic safeguards, because of the correlation result between paper method and national standard method, and the characteristics of simple operation, which is suitable for on-site detection. However, the paper method is still not an alternative to the national standard method.

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