应用小鼠局部淋巴结ATP检测法检测染发剂刺激性

Evaluation on Skin Irritation of Hair Dyes with Modified Local Lymph Node Assay

摘要:
目的对比小鼠局部淋巴结法ATP检测法（LLNA：DA）和经典皮肤刺激实验，评价两方法检测染发剂刺激性的能力。
方法市场随机购买15种不同牌子染发剂，按照OECD442（A）和OECD404标准方法，对15种染发剂进行刺激性检测。
结果 LLNA：DA法中3号、4号、5号、6号、11号、13号和15号染发剂具有刺激性；多次皮肤刺激实验中3号、7号、和13号具有轻刺激性，4号和11号具有中刺激性。
结论 LLNA：DA法检测染发剂刺激性的能力与多次皮肤刺激实验相当，值得深入探讨研究。

Abstract:
Objectives To compare a murine local lymph node assay (LLNA):DA method and the classical dermal irritation method to evaluate the dermal irritation of hair dyes, and evaluate their ability of detecting the irritation of hair dyes.
Methods Fifteen kinds of hair colorants were randomly selected. The sensitization of 15 kinds of hair dyes were examined according to OECD442 (A) and OECD442 (B) standard method.
Results Samples No.3, 4, 5, 6, 11, 13 and 15 showed a positive irritation tested by LLNA:DA method. Samples No.3, 4 and 5 showed a light positive irritation and samples No.11 and 13 showed a moderate positive irritation tested by dermal irritation test.
Conclusions The sensitivity of LLNA:DA method for evaluating the dermal irritation of hair dyes was equal to that of classical dermal irritation test.