电感耦合等离子体发射光谱法测定人血浆中5种金属元素

武鑫; 赵玮; 宋楠楠; 闫慧芳

doi:10.13421/j.cnki.hjwsxzz.2018.02.011

电感耦合等离子体发射光谱法测定人血浆中5种金属元素

Simultaneous Determination of Five Metals in Human Plasma by Inductively Coupled Plasma Optical Emission Spectrometry

摘要

摘要:
目的建立人血浆中锌、铁、镁、钙、铜5种金属元素的电感耦合等离子体发射光谱（ICP-OES）测定方法。
方法采用1%（v/v）硝酸对血浆进行10倍稀释后直接进行ICP-OES分析，标准曲线法定量。
结果 5种金属元素在对应的浓度范围内线性良好，相关系数均>0.999，方法检出限为（0.004 1~0.015 1）mg/L。方法批内精密度（RSD%）为0.8%~2.5%，方法批间精密度（RSD%）为1.4%~3.0%，平均加标回收率范围为93.3%~105.2%；血清标准物质中5种元素测定结果均在参考值范围内。
结论建立的方法可用于人血浆中5种金属元素的测定。

Abstract:
Objectives To establish an inductively coupled plasma optical emission spectrometry (ICP-OES) for the determination of 5 metals, including Zn, Fe, Mg, Ca and Cu in human plasma.
Methods Plasma samples were analyzed by ICP-OES after 10 times dilution with 1% (v/v) nitric acid solution and quantified by standard curve method.
Results There were good linearity of standard curves in the detection range of (0.004 1~0.015 1) mg/L for 5 metals, with a correlation coefficient of >0.999. The intra-assay precision of 5 metals were 0.8%~2.5% and the inter-assay precision of 5 metal were 1.4%~3.0%. The spiked for all elements was in the range of 93.3%~105.2%. The result of five elements in the reference materials were in the acceptable ranges.
Conclusions The inductively coupled plasma optical emission spectrometry (ICP-OES) method could be used to monitor the concentrations of the 5 metals in human plasma.

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