ICP-MS同时测定全血中多种微量元素

赵立凡; 唐宏兵; 欧阳运富; 曹毅; 吉俊敏

doi:10.13421/j.cnki.hjwsxzz.2017.05.015

ICP-MS同时测定全血中多种微量元素

Simultaneous Determination of Micro Elements in Whole Blood by ICP-MS

摘要

摘要:
目的建立碰撞反应模式ICP-MS同时测定全血中多种微量元素的准确分析方法。
方法取少量血液样品经浓硝酸水浴消解后，采用电感耦合等离子体质谱（CRI-ICP-MS）法对全血中微量元素进行分析，通过在线加入内标校正基体效应和信号漂移，应用碰撞反应模式消除质量数双原子干扰。采用质量控制样品的相应元素进行质量控制，检测测定结果和推荐值是否吻合。
结果各微量元素方法检出限在（0.017~0.83）μg/L之间，相对标准偏差（RSD） < 10%，加标回收率为87.3%~109.8%。
结论方法具有简单、快速和准确的特点，可用ICP-MS直接测定全血液中的微量元素。

Abstract:
Objective To develop an accurate method for simultaneous determination of trace micro elements in whole blood by inductively coupled plasma mass spectrometry with collision reaction interface (CRI-ICP-MS).
Methods Micro amount of blood sample was digested with concentrated nitric acid on a water bath, then the micro elements in blood were determined by collision reaction interface ICP-MS. The matrix effects and signal drift were corrected by the online addition of internal standards, and the interference of elements was eliminated by the application of collision reaction interface technology. The accuracy of the method was evaluated by the agreement of the result with the certified value of standard reference sample.
Result The detection limit was in the range of (0.017~0.83)μg/L, the relative standard deviation was less than 10%, and the recovery of standard addition was in the range of 87.3%~109.8%.
Conclusion This method is simple, sensitive and accurate, which can be used to determine trace micro elements in whole blood directly.

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