苦味酸分光光度法现场快速测定尿中肌酐

张淼; 郑磊; 周志荣; 丁亮; 林少彬

doi:10.13421/j.cnki.hjwsxzz.2016.04.008

苦味酸分光光度法现场快速测定尿中肌酐

A Rapid Method for the Determination of Urinary Creatinine by the Picric Acid Spectrophotometry

摘要

摘要:
目的建立尿中肌酐的现场快速测定法。
方法取尿样20.0 μL于10 mL离心管中，用瓶口分配器加入3.02 mL纯水，再加入500 μL显色液，于490 nm下，以样品空白溶液校零，测定尿样肌酐含量。
结果本方法在0~2.00 g/L浓度呈现良好的线性关系，线性回归方程为C=1.5857*A-0.0112，相关系数达到0.9998，检出限为0.005 g/L，低、中和高三个浓度平均加标回收率在98.6%~99.6%之间，相对标准偏差在1.6%~2.5%之间(n=6)。在测定的613份尿样中，测定范围在0.08 g/L~4.44 g/L之间。
结论本方法操作简便快速、准确性好、灵敏度高，可用于体检现场快速测定尿肌酐含量。

Abstract:
Objective To establish a rapid method for the determination of creatinine in urine samples.
Methods A volume of 20.0 μL urine sample in a 10 mL centrifuge tube was diluted with 3.02 mL pure water. After adding 500 μL chromogenic agent and standing for 15 min, the content of creatinine was measured by the absorbance at 490 nm with a blank for zero adjustment.
Results The linear range of this method was 0 g/L~2.00 g/L. The regression equation was C=1.5857*A-0.0112 and the correlation coefficient was 0.9998. The detection limit, recovery and relative standard deviation was 0.005 g/L, 96.9%~104% and 1.6%~2.5%, respectively. The content of creatinine in 613 urine samples tested by this method was in the range of 0.08 g/L~4.44 g/L.
Conclusion The method was rapid, accurate, sensitive, easier to operate and could be used for the determination of urinary creatinine in the field for health checkup.

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